How To Identify Genes Using cDNA Library

Question

Imagine that you are a scientist and you have just discovered a protein in jellyfish that gives off green light. You don’t know the gene from which the protein is made but you do know the first ten amino acids of the protein (assume the jellyfish genome has not been sequenced). How could you use a cDNA library to identify the gene and make large amounts of the protein in bacteria? Please include all the steps you would take and what tools (enzymes, plasmids, etc..) you would use to accomplish this. You can describe this or draw a labeled diagram.

 

Summary

The question belongs to Biology and it discusses about using cDNA library for identifying genes and making large amounts of a protein in a bacteria.

Total Word Count 430

Download Full Solution

Comments

  • HWA
    Rasha

    this is a very good website

  • HWA
    maani

    I have 50 questions for the same test your page is showing only 28

  • HWA
    joeanne

    hi can you please help or guide me to answer my assignments. thanks

  • HWA
    joeanne

    hi can anyone help or guide me to my assignments. thanks

  • HWA
    Monik


  • HWA
    Cristina

    This solution is perfect ...thanks

  • HWA
    Janete

    Hello Allison,I love the 2nd image that you did! I also, had never heard of SumoPaint, is something that I will have to exolpre a bit! I understand completely the 52 (or so) youtube videos that you probably watched. Sometimes they have what you want, sometimes they don't! However, it is always satisfying when you are able to produce something that you have taught yourself. Great job!Debra 0 likes

  • HWA
    Sandeep

    Perfect bank of solution. 

  • HWA
    Oxana

    great !

  • HWA
    Paul Brandon-Fritzius

    thanks for the quick response. the solution looks good. :)

  • HWA
    tina Johnson

    thnx for the answer. it was perfect. just the way i wanted it. 

  • HWA
    Giuseppe

    works fine.