You receive a freshly prepared slide of a liver tissue that a lab colleague prepared with an H&E protocol. Upon placing it under the microscope you notice that the integrity of the cells are perfectly conserved but that there is close to no contrast throughout the slide. Your colleague is 100% certain that he used the correct dyes and you trust his certainty on that matter. Considering the above information, what did your lab mate do wrong?
The question belongs to Biology and it discusses about a scenario of H&E protocol about a slide of liver tissue.
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