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Determination of YX/S,obs in Bioreactors


Consider the growth of enterobacter aerogenes in each of two independent chemostat operations, each of volume 500 L, each operating at steady state. Cell maintenance effects in each bioreactor are negligible, and product formation in each bioreactor is negligible. Growth of E,  aerogenes in each bioreactor is limited by ammonium ion, such that

Mass transfer limitations in each bioreactor are negligible. Finally, in each bioreactor, the feed is sterile and inlet flow rates are identical with S0 = 4.8 g/L, but substrate conversions are not the same.

Now suppose cell maintenance effects are not negligible, such that maintenance is achieved entirely by endogenous metabolism, with Kd = 0.030 h-1.

Determine whether YX/S,obs in bioreactor 1 would be less than, equal to, or greater than YX/S,obs in bioreactor 2.


This question belongs to bioreactors in biotechnology and discusses about bioreactors and to determine YX/S,obs in bioreactors.

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