## Solution Library

# Determination of Ammonia Conversions and Cell Concentrations

**Question**

Consider the growth of enterobacter aerogenes in each of two independent chemostat operations, each of volume 500 L, each operating at steady state. Cell maintenance effects in each bioreactor are negligible, and product formation in each bioreactor is negligible. Growth of E, aerogenes in each bioreactor is limited by ammonium ion, such that

Mass transfer limitations in each bioreactor are negligible. Finally, in each bioreactor, the feed is sterile and inlet flow rates are identical with S_{0} = 4.8 g/L, but substrate conversions are not the same.

- If ammonia conversion in bioreactor 1 is 70%, determine the ammonium conversion in bioreactor 2.
- Determine whether the cell concentration in bioreactor 1 would be less than, equal to, or greater than the cell concentration in bioreactor 2.
- Determine whether Y
_{X/S,obs}in bioreactor 1 would be less than, equal to, or greater than Y_{X/S,obs}in bioreactor 2. - Determine whether Y
_{X/S,obs}in either bioreactor would be expected to change with a change in flow rate.

Now suppose cell maintenance effects are not negligible, such that maintenance is achieved entirely by endogenous metabolism, with K_{d} = 0.030 h^{-1}.

- If ammonium conversion in bioreactor 1 is 70%, determine the ammonium conversion in bioreactor 2.
- Determine whether the cell concentration in bioreactor 1 would be less than, equal to, or greater than the cell concentration in bioreactor 2.
- Determine whether Y
_{X/S,obs}in bioreactor 1 would be less than, equal to, or greater than Y_{X/S,obs}in bioreactor 2. - Determine whether Y
_{X/S,obs}in either bioreactor would be expected to change with a change in flow rate.

**Summary**

This question belongs to bioreactors in biotechnology and discusses about bioreactors and to determine different reactions and concentrations related to bioreactors.

Total word count: image

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